The influence of environmental calcium concentrations on calcium flux, compensatory drinking and epithelial calcium channel expression in a freshwater cartilaginous fish.
Identifieur interne : 000572 ( Main/Exploration ); précédent : 000571; suivant : 000573The influence of environmental calcium concentrations on calcium flux, compensatory drinking and epithelial calcium channel expression in a freshwater cartilaginous fish.
Auteurs : Peter J. Allen [Canada] ; Dirk Weihrauch ; Vanessa Grandmaison ; Patricia Dasiewicz ; Stephan J. Peake ; W Gary AndersonSource :
- The Journal of experimental biology [ 1477-9145 ] ; 2011.
English descriptors
- KwdEn :
- Acclimatization (drug effects), Amino Acid Sequence, Animals, Base Sequence, Calcium (blood), Calcium (metabolism), Calcium (pharmacology), Calcium Channels (chemistry), Calcium Channels (genetics), Calcium Channels (metabolism), Cartilage (drug effects), Cartilage (metabolism), Drinking Behavior (drug effects), Environment, Epithelial Cells (drug effects), Epithelial Cells (metabolism), Fishes (blood), Fishes (physiology), Fresh Water, Gene Expression Regulation (drug effects), Gills (drug effects), Gills (metabolism), Intestines (drug effects), Intestines (metabolism), Molecular Sequence Data, Peptide Elongation Factor 1 (metabolism), Phylogeny, RNA, Messenger (genetics), RNA, Messenger (metabolism), Reverse Transcriptase Polymerase Chain Reaction.
- MESH :
- chemical , blood : Calcium.
- chemical , chemistry : Calcium Channels.
- blood : Fishes.
- drug effects : Acclimatization, Cartilage, Drinking Behavior, Epithelial Cells, Gene Expression Regulation, Gills, Intestines.
- chemical , genetics : Calcium Channels, RNA, Messenger.
- chemical , metabolism : Calcium, Calcium Channels, Cartilage, Epithelial Cells, Gills, Intestines, Peptide Elongation Factor 1, RNA, Messenger.
- chemical , pharmacology : Calcium.
- physiology : Fishes.
- Amino Acid Sequence, Animals, Base Sequence, Environment, Fresh Water, Molecular Sequence Data, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Calcium metabolism and mRNA levels of the epithelial calcium channel (ECaC) were examined in a freshwater cartilaginous fish, the lake sturgeon Acipenser fulvescens. Lake sturgeon were acclimated for ≥2 weeks to 0.1 (low), 0.4 (normal) or 3.3 (high) mmol l(-1) environmental calcium. Whole-body calcium flux was examined using (45)Ca as a radioactive marker. Net calcium flux was inward in all treatment groups; however, calcium influx was greatest in the low calcium environment and lowest in the high calcium environment, whereas efflux had the opposite relationship. A significant difference in the concentration of (45)Ca in the gastrointestinal tract (GIT) of fish in the low calcium environment led to the examination of drinking rate and calcium flux across the anterior-middle (mid) intestine. Drinking rate was not different between treatments; however, calcium influx across the mid-intestine in the low calcium treatment was significantly greater than that in both the normal and high calcium treatments. The lake sturgeon ECaC was 2831 bp in length, with a predicted protein sequence of 683 amino acids that shared a 66% identity with the closest sequenced ECaCs from the vertebrate phyla. ECaC mRNA levels were examined in the gills, kidney, pyloric caeca, mid-intestine and spiral intestine. Expression levels were highest in the gills, then the kidneys, and were orders of magnitude lower in the GIT. Contrary to existing models for calcium uptake in the teleost gill, ECaC expression was greatest in high calcium conditions and kidney ECaC expression was lowest in low calcium conditions, suggesting that cellular transport mechanisms for calcium may be distinctly different in these freshwater cartilaginous fishes.
DOI: 10.1242/jeb.041087
PubMed: 21346128
Affiliations:
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Allen</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, University of Manitoba, Winnipeg, MB, Canada. pallen@cfr.msstate.edu</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biological Sciences, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Weihrauch, Dirk" sort="Weihrauch, Dirk" uniqKey="Weihrauch D" first="Dirk" last="Weihrauch">Dirk Weihrauch</name></author><author><name sortKey="Grandmaison, Vanessa" sort="Grandmaison, Vanessa" uniqKey="Grandmaison V" first="Vanessa" last="Grandmaison">Vanessa Grandmaison</name></author><author><name sortKey="Dasiewicz, Patricia" sort="Dasiewicz, Patricia" uniqKey="Dasiewicz P" first="Patricia" last="Dasiewicz">Patricia Dasiewicz</name></author><author><name sortKey="Peake, Stephan J" sort="Peake, Stephan J" uniqKey="Peake S" first="Stephan J" last="Peake">Stephan J. Peake</name></author><author><name sortKey="Anderson, W Gary" sort="Anderson, W Gary" uniqKey="Anderson W" first="W Gary" last="Anderson">W Gary Anderson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21346128</idno><idno type="pmid">21346128</idno><idno type="doi">10.1242/jeb.041087</idno><idno type="wicri:Area/PubMed/Corpus">000362</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000362</idno><idno type="wicri:Area/PubMed/Curation">000362</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000362</idno><idno type="wicri:Area/PubMed/Checkpoint">000362</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000362</idno><idno type="wicri:Area/Ncbi/Merge">000371</idno><idno type="wicri:Area/Ncbi/Curation">000371</idno><idno type="wicri:Area/Ncbi/Checkpoint">000371</idno><idno type="wicri:Area/Main/Merge">000581</idno><idno type="wicri:Area/Main/Curation">000572</idno><idno type="wicri:Area/Main/Exploration">000572</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The influence of environmental calcium concentrations on calcium flux, compensatory drinking and epithelial calcium channel expression in a freshwater cartilaginous fish.</title><author><name sortKey="Allen, Peter J" sort="Allen, Peter J" uniqKey="Allen P" first="Peter J" last="Allen">Peter J. Allen</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, University of Manitoba, Winnipeg, MB, Canada. pallen@cfr.msstate.edu</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biological Sciences, University of Manitoba, Winnipeg, MB</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Weihrauch, Dirk" sort="Weihrauch, Dirk" uniqKey="Weihrauch D" first="Dirk" last="Weihrauch">Dirk Weihrauch</name></author><author><name sortKey="Grandmaison, Vanessa" sort="Grandmaison, Vanessa" uniqKey="Grandmaison V" first="Vanessa" last="Grandmaison">Vanessa Grandmaison</name></author><author><name sortKey="Dasiewicz, Patricia" sort="Dasiewicz, Patricia" uniqKey="Dasiewicz P" first="Patricia" last="Dasiewicz">Patricia Dasiewicz</name></author><author><name sortKey="Peake, Stephan J" sort="Peake, Stephan J" uniqKey="Peake S" first="Stephan J" last="Peake">Stephan J. Peake</name></author><author><name sortKey="Anderson, W Gary" sort="Anderson, W Gary" uniqKey="Anderson W" first="W Gary" last="Anderson">W Gary Anderson</name></author></analytic><series><title level="j">The Journal of experimental biology</title><idno type="eISSN">1477-9145</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acclimatization (drug effects)</term><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Calcium (blood)</term><term>Calcium (metabolism)</term><term>Calcium (pharmacology)</term><term>Calcium Channels (chemistry)</term><term>Calcium Channels (genetics)</term><term>Calcium Channels (metabolism)</term><term>Cartilage (drug effects)</term><term>Cartilage (metabolism)</term><term>Drinking Behavior (drug effects)</term><term>Environment</term><term>Epithelial Cells (drug effects)</term><term>Epithelial Cells (metabolism)</term><term>Fishes (blood)</term><term>Fishes (physiology)</term><term>Fresh Water</term><term>Gene Expression Regulation (drug effects)</term><term>Gills (drug effects)</term><term>Gills (metabolism)</term><term>Intestines (drug effects)</term><term>Intestines (metabolism)</term><term>Molecular Sequence Data</term><term>Peptide Elongation Factor 1 (metabolism)</term><term>Phylogeny</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Calcium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Calcium Channels</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Acclimatization</term><term>Cartilage</term><term>Drinking Behavior</term><term>Epithelial Cells</term><term>Gene Expression Regulation</term><term>Gills</term><term>Intestines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Calcium Channels</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term><term>Calcium Channels</term><term>Cartilage</term><term>Epithelial Cells</term><term>Gills</term><term>Intestines</term><term>Peptide Elongation Factor 1</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Calcium</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Environment</term><term>Fresh Water</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Calcium metabolism and mRNA levels of the epithelial calcium channel (ECaC) were examined in a freshwater cartilaginous fish, the lake sturgeon Acipenser fulvescens. Lake sturgeon were acclimated for ≥2 weeks to 0.1 (low), 0.4 (normal) or 3.3 (high) mmol l(-1) environmental calcium. Whole-body calcium flux was examined using (45)Ca as a radioactive marker. Net calcium flux was inward in all treatment groups; however, calcium influx was greatest in the low calcium environment and lowest in the high calcium environment, whereas efflux had the opposite relationship. A significant difference in the concentration of (45)Ca in the gastrointestinal tract (GIT) of fish in the low calcium environment led to the examination of drinking rate and calcium flux across the anterior-middle (mid) intestine. Drinking rate was not different between treatments; however, calcium influx across the mid-intestine in the low calcium treatment was significantly greater than that in both the normal and high calcium treatments. The lake sturgeon ECaC was 2831 bp in length, with a predicted protein sequence of 683 amino acids that shared a 66% identity with the closest sequenced ECaCs from the vertebrate phyla. ECaC mRNA levels were examined in the gills, kidney, pyloric caeca, mid-intestine and spiral intestine. Expression levels were highest in the gills, then the kidneys, and were orders of magnitude lower in the GIT. Contrary to existing models for calcium uptake in the teleost gill, ECaC expression was greatest in high calcium conditions and kidney ECaC expression was lowest in low calcium conditions, suggesting that cellular transport mechanisms for calcium may be distinctly different in these freshwater cartilaginous fishes.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><noCountry><name sortKey="Anderson, W Gary" sort="Anderson, W Gary" uniqKey="Anderson W" first="W Gary" last="Anderson">W Gary Anderson</name><name sortKey="Dasiewicz, Patricia" sort="Dasiewicz, Patricia" uniqKey="Dasiewicz P" first="Patricia" last="Dasiewicz">Patricia Dasiewicz</name><name sortKey="Grandmaison, Vanessa" sort="Grandmaison, Vanessa" uniqKey="Grandmaison V" first="Vanessa" last="Grandmaison">Vanessa Grandmaison</name><name sortKey="Peake, Stephan J" sort="Peake, Stephan J" uniqKey="Peake S" first="Stephan J" last="Peake">Stephan J. Peake</name><name sortKey="Weihrauch, Dirk" sort="Weihrauch, Dirk" uniqKey="Weihrauch D" first="Dirk" last="Weihrauch">Dirk Weihrauch</name></noCountry><country name="Canada"><region name="Manitoba"><name sortKey="Allen, Peter J" sort="Allen, Peter J" uniqKey="Allen P" first="Peter J" last="Allen">Peter J. Allen</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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